Assessment of the Agreement between Fosters and Trainers Evaluating Behavior with the C-BARQ and Behavior Checklist

class: top, title

.left-col-narrow[.center[
<br>

<a href="https://www.iwdba.org/event/iwdc-2021/"><img src="img/iwdbaLogo.png" alt="International Working Dog Breeding Association" width="80%"/></a>
.titleConf[IWDC 2021]
]]

.right-col-wide[
.left[
# Assessment of the Agreement between Fosters and Trainers Evaluating Behavior with the C-BARQ and Behavior Checklist

----
]]

.titleAuthor[
Elizabeth Hare PhD<br>@DogGeneticsLLC <svg aria-label="Twitter" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Twitter</title><path d="M459.37 151.716c.325 4.548.325 9.097.325 13.645 0 138.72-105.583 298.558-298.558 298.558-59.452 0-114.68-17.219-161.137-47.106 8.447.974 16.568 1.299 25.34 1.299 49.055 0 94.213-16.568 130.274-44.832-46.132-.975-84.792-31.188-98.112-72.772 6.498.974 12.995 1.624 19.818 1.624 9.421 0 18.843-1.3 27.614-3.573-48.081-9.747-84.143-51.98-84.143-102.985v-1.299c13.969 7.797 30.214 12.67 47.431 13.319-28.264-18.843-46.781-51.005-46.781-87.391 0-19.492 5.197-37.36 14.294-52.954 51.655 63.675 129.3 105.258 216.365 109.807-1.624-7.797-2.599-15.918-2.599-24.04 0-57.828 46.782-104.934 104.934-104.934 30.213 0 57.502 12.67 76.67 33.137 23.715-4.548 46.456-13.32 66.599-25.34-7.798 24.366-24.366 44.833-46.132 57.827 21.117-2.273 41.584-8.122 60.426-16.243-14.292 20.791-32.161 39.308-52.628 54.253z"/></svg>
]

---
class: bottom, center

.pull-left[
<img style="border-radius: 50%;" 
src="img/Profile_LH.JPG",
alt="Photo of Liz Hare with an elderly Golden Retriever and a young Labrador Retriever in harness",
width="250px"
/>

# Elizabeth Hare PhD

[<svg aria-label="Link" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Link</title><path d="M326.612 185.391c59.747 59.809 58.927 155.698.36 214.59-.11.12-.24.25-.36.37l-67.2 67.2c-59.27 59.27-155.699 59.262-214.96 0-59.27-59.26-59.27-155.7 0-214.96l37.106-37.106c9.84-9.84 26.786-3.3 27.294 10.606.648 17.722 3.826 35.527 9.69 52.721 1.986 5.822.567 12.262-3.783 16.612l-13.087 13.087c-28.026 28.026-28.905 73.66-1.155 101.96 28.024 28.579 74.086 28.749 102.325.51l67.2-67.19c28.191-28.191 28.073-73.757 0-101.83-3.701-3.694-7.429-6.564-10.341-8.569a16.037 16.037 0 0 1-6.947-12.606c-.396-10.567 3.348-21.456 11.698-29.806l21.054-21.055c5.521-5.521 14.182-6.199 20.584-1.731a152.482 152.482 0 0 1 20.522 17.197zM467.547 44.449c-59.261-59.262-155.69-59.27-214.96 0l-67.2 67.2c-.12.12-.25.25-.36.37-58.566 58.892-59.387 154.781.36 214.59a152.454 152.454 0 0 0 20.521 17.196c6.402 4.468 15.064 3.789 20.584-1.731l21.054-21.055c8.35-8.35 12.094-19.239 11.698-29.806a16.037 16.037 0 0 0-6.947-12.606c-2.912-2.005-6.64-4.875-10.341-8.569-28.073-28.073-28.191-73.639 0-101.83l67.2-67.19c28.239-28.239 74.3-28.069 102.325.51 27.75 28.3 26.872 73.934-1.155 101.96l-13.087 13.087c-4.35 4.35-5.769 10.79-3.783 16.612 5.864 17.194 9.042 34.999 9.69 52.721.509 13.906 17.454 20.446 27.294 10.606l37.106-37.106c59.271-59.259 59.271-155.699.001-214.959z"/></svg> doggenetics.com](http://doggenetics.com/)<br>
[<svg aria-label="Twitter" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Twitter</title><path d="M459.37 151.716c.325 4.548.325 9.097.325 13.645 0 138.72-105.583 298.558-298.558 298.558-59.452 0-114.68-17.219-161.137-47.106 8.447.974 16.568 1.299 25.34 1.299 49.055 0 94.213-16.568 130.274-44.832-46.132-.975-84.792-31.188-98.112-72.772 6.498.974 12.995 1.624 19.818 1.624 9.421 0 18.843-1.3 27.614-3.573-48.081-9.747-84.143-51.98-84.143-102.985v-1.299c13.969 7.797 30.214 12.67 47.431 13.319-28.264-18.843-46.781-51.005-46.781-87.391 0-19.492 5.197-37.36 14.294-52.954 51.655 63.675 129.3 105.258 216.365 109.807-1.624-7.797-2.599-15.918-2.599-24.04 0-57.828 46.782-104.934 104.934-104.934 30.213 0 57.502 12.67 76.67 33.137 23.715-4.548 46.456-13.32 66.599-25.34-7.798 24.366-24.366 44.833-46.132 57.827 21.117-2.273 41.584-8.122 60.426-16.243-14.292 20.791-32.161 39.308-52.628 54.253z"/></svg> @DogGeneticsLLC](https://twitter.com/DogGeneticsLLC)<br>
[<svg aria-label="GitHub" role="img" viewBox="0 0 496 512" style="height:1em;width:0.97em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>GitHub</title><path d="M165.9 397.4c0 2-2.3 3.6-5.2 3.6-3.3.3-5.6-1.3-5.6-3.6 0-2 2.3-3.6 5.2-3.6 3-.3 5.6 1.3 5.6 3.6zm-31.1-4.5c-.7 2 1.3 4.3 4.3 4.9 2.6 1 5.6 0 6.2-2s-1.3-4.3-4.3-5.2c-2.6-.7-5.5.3-6.2 2.3zm44.2-1.7c-2.9.7-4.9 2.6-4.6 4.9.3 2 2.9 3.3 5.9 2.6 2.9-.7 4.9-2.6 4.6-4.6-.3-1.9-3-3.2-5.9-2.9zM244.8 8C106.1 8 0 113.3 0 252c0 110.9 69.8 205.8 169.5 239.2 12.8 2.3 17.3-5.6 17.3-12.1 0-6.2-.3-40.4-.3-61.4 0 0-70 15-84.7-29.8 0 0-11.4-29.1-27.8-36.6 0 0-22.9-15.7 1.6-15.4 0 0 24.9 2 38.6 25.8 21.9 38.6 58.6 27.5 72.9 20.9 2.3-16 8.8-27.1 16-33.7-55.9-6.2-112.3-14.3-112.3-110.5 0-27.5 7.6-41.3 23.6-58.9-2.6-6.5-11.1-33.3 2.6-67.9 20.9-6.5 69 27 69 27 20-5.6 41.5-8.5 62.8-8.5s42.8 2.9 62.8 8.5c0 0 48.1-33.6 69-27 13.7 34.7 5.2 61.4 2.6 67.9 16 17.7 25.8 31.5 25.8 58.9 0 96.5-58.9 104.2-114.8 110.5 9.2 7.9 17 22.9 17 46.4 0 33.7-.3 75.4-.3 83.6 0 6.5 4.6 14.4 17.3 12.1C428.2 457.8 496 362.9 496 252 496 113.3 383.5 8 244.8 8zM97.2 352.9c-1.3 1-1 3.3.7 5.2 1.6 1.6 3.9 2.3 5.2 1 1.3-1 1-3.3-.7-5.2-1.6-1.6-3.9-2.3-5.2-1zm-10.8-8.1c-.7 1.3.3 2.9 2.3 3.9 1.6 1 3.6.7 4.3-.7.7-1.3-.3-2.9-2.3-3.9-2-.6-3.6-.3-4.3.7zm32.4 35.6c-1.6 1.3-1 4.3 1.3 6.2 2.3 2.3 5.2 2.6 6.5 1 1.3-1.3.7-4.3-1.3-6.2-2.2-2.3-5.2-2.6-6.5-1zm-11.4-14.7c-1.6 1-1.6 3.6 0 5.9 1.6 2.3 4.3 3.3 5.6 2.3 1.6-1.3 1.6-3.9 0-6.2-1.4-2.3-4-3.3-5.6-2z"/></svg> @LizHareDogs](https://github.com/LizHareDogs)<br>
[<svg aria-label="Envelope" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Envelope</title><path d="M502.3 190.8c3.9-3.1 9.7-.2 9.7 4.7V400c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V195.6c0-5 5.7-7.8 9.7-4.7 22.4 17.4 52.1 39.5 154.1 113.6 21.1 15.4 56.7 47.8 92.2 47.6 35.7.3 72-32.8 92.3-47.6 102-74.1 131.6-96.3 154-113.7zM256 320c23.2.4 56.6-29.2 73.4-41.4 132.7-96.3 142.8-104.7 173.4-128.7 5.8-4.5 9.2-11.5 9.2-18.9v-19c0-26.5-21.5-48-48-48H48C21.5 64 0 85.5 0 112v19c0 7.4 3.4 14.3 9.2 18.9 30.6 23.9 40.7 32.4 173.4 128.7 16.8 12.2 50.2 41.8 73.4 41.4z"/></svg> LizHare@DogGenetics.com](mailto:LizHare@DogGenetics.com)
]

.pull-right[

## Collaborators

.large[Jennifer L. Essler PhD]

.large[Cynthia M. Otto DVM PhD DACVECC DACVSMR]

.large[James A. Serpell PhD]

]

---
# Sources for PennVet Working Dog Center Pups

.pull-left[
- Working Dog Center Detector Dog Breeding Program
    - Labrador Retrievers
	- Dutch Shepherds  
	
- Breeder donations
    -  Pups from litters with working parents and relatives
	- Health standards for parents
    - Herding and sporting breeds]
	
.pull-right[
<img src="img/IMG_5840.jpeg" title="An ADORABLE baby black lab gnaws on a green and purple chew toy, looking up at the camera at an angle.  It is in a crate outdoors sitting on grass." alt="An ADORABLE baby black lab gnaws on a green and purple chew toy, looking up at the camera at an angle.  It is in a crate outdoors sitting on grass." width="164" height="90%" style="display: block; margin: auto;" />
]

---
# Puppy Development at the PennVet Working Dog Center

.pull-left[
- Pups live with foster families
- Training begins ~8 weeks of age
  - [Fit to Work](https://www.frontiersin.org/articles/10.3389/fvets.2020.00470/full)
  - Obedience
  - Agility
  - Search]
  
  .pull-right[
<img src="img/BretBooks.png" title="A reddish Golden is lying in sphinx position on a large rectangular trampoline in a big room.  Its front paws are resting on an open book, and the position of the dog’s head makes it look like it is reading.  There are several other books piled on the floor around the trampoline." alt="A reddish Golden is lying in sphinx position on a large rectangular trampoline in a big room.  Its front paws are resting on an open book, and the position of the dog’s head makes it look like it is reading.  There are several other books piled on the floor around the trampoline." width="90%" style="display: block; margin: auto;" />
]
---
# Intensive Interaction and Observation

.pull-left[
- Career choice based on
  - Formal assessment
  - Trainer observation
- Career flexibility contributes to very high success rate: 93%]

.pull-right[
<img src="img/IMG_6611.JPG" title="In a large room there are two red utility ladders with a plank resting on them approximately 4ft above the ground.  A straight ladder is resting on the plank, at an angle.  A black lab is standing on the plank, looking the camera as a blond woman with short hair and glasses on top of her head, smiles up at the dog. The ladders have “Penn Vet Working Dog Center” bumper stickers stuck on the uprights." alt="In a large room there are two red utility ladders with a plank resting on them approximately 4ft above the ground.  A straight ladder is resting on the plank, at an angle.  A black lab is standing on the plank, looking the camera as a blond woman with short hair and glasses on top of her head, smiles up at the dog. The ladders have “Penn Vet Working Dog Center” bumper stickers stuck on the uprights." width="90%" style="display: block; margin: auto;" />
]
---
# Formal Assessments
- Behavior questionnaires
  - BCL (Behavior Checklist) is completed by trainer
  - C-BARQ (Canine Behavioral and Research Questionnaire) is completed by foster
- Behavior tests withs Scoring
  - Engagement
  - Environment

---
# C-BARQ

- 100 items
- Most items cluster into one of 14 subscales
- Some miscellaneous items do not cluster
- Problem behaviors, except trainability

---
# C-BARQ Scoring

<table class="table" style="width: auto !important; margin-left: auto; margin-right: auto;">
<caption>Behaviors Rated 0 to 4 on either Frequency or Intensity</caption>
 <thead>
  <tr>
   <th style="text-align:right;"> Score </th>
   <th style="text-align:left;"> Frequency </th>
   <th style="text-align:left;"> Intensity </th>
  </tr>
 </thead>
<tbody>
  <tr>
   <td style="text-align:right;"> 0 </td>
   <td style="text-align:left;"> Never </td>
   <td style="text-align:left;"> None </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 1 </td>
   <td style="text-align:left;"> Seldom </td>
   <td style="text-align:left;"> Mild </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 2 </td>
   <td style="text-align:left;"> Sometimes </td>
   <td style="text-align:left;"> Moderate </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 3 </td>
   <td style="text-align:left;"> Usually </td>
   <td style="text-align:left;"> Moderate to Extreme </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 4 </td>
   <td style="text-align:left;"> Always </td>
   <td style="text-align:left;"> Extreme </td>
  </tr>
</tbody>
</table>

Each subscale score is the mean of the items in the subscale.

Subscale scores were considered missing when less than 70% of items had responses

---

# BCL

.pull-left[
- 43 individual items
- no clusters
- most BCL items overlap with C-BARQ subscales]

.pull-right[
<table class="table" style="width: auto !important; margin-left: auto; margin-right: auto;">
<caption>Behaviors Rated 0 to 4 on Intensity</caption>
 <thead>
  <tr>
   <th style="text-align:right;"> Score </th>
   <th style="text-align:left;"> Intensity </th>
  </tr>
 </thead>
<tbody>
  <tr>
   <td style="text-align:right;"> 0 </td>
   <td style="text-align:left;"> Absent </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 1 </td>
   <td style="text-align:left;"> Very Mild </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 2 </td>
   <td style="text-align:left;"> Mild </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 3 </td>
   <td style="text-align:left;"> Moderate </td>
  </tr>
  <tr>
   <td style="text-align:right;"> 4 </td>
   <td style="text-align:left;"> Severe </td>
  </tr>
</tbody>
</table>
]

---
# Dogs

.pull-left[
Assigned to age group categories:
- up to 9 months
- 9 months to 2 years
- 2 years and up

Randomly retained one of each assessment per dog per age category]

.pull-right[
<table class="table" style="margin-left: auto; margin-right: auto;">
 <thead>
  <tr>
   <th style="text-align:left;"> Age Category </th>
   <th style="text-align:right;"> N </th>
  </tr>
 </thead>
<tbody>
  <tr>
   <td style="text-align:left;"> Up to 9 mo </td>
   <td style="text-align:right;"> 39 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> 9 mo to 2 y </td>
   <td style="text-align:right;"> 64 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Over 2 y </td>
   <td style="text-align:right;"> 5 </td>
  </tr>
</tbody>
</table>
]
---

# BCL - C-BARQ Correlations

How closely do BCL and C-BARQ scores correlate?

Two analyses of Spearman correlation with Holm adjustment for multiple testing
1. BCL and C-BARQ subscales
2. BCL and C-BARQ individual items

---
# BCL items and C-BARQ subscales: Correlations

<table class="table" style="width: auto !important; margin-left: auto; margin-right: auto;">
 <thead>
  <tr>
   <th style="text-align:left;"> BCL </th>
   <th style="text-align:left;"> C-BARQ </th>
   <th style="text-align:right;"> rho </th>
   <th style="text-align:left;"> p </th>
  </tr>
 </thead>
<tbody>
  <tr>
   <td style="text-align:left;"> Stranger-directed aggression </td>
   <td style="text-align:left;"> Stranger-directed aggression </td>
   <td style="text-align:right;"> 0.49 </td>
   <td style="text-align:left;"> &lt; 0.001 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Stranger-directed aggression </td>
   <td style="text-align:left;"> Dog-directed aggression </td>
   <td style="text-align:right;"> 0.42 </td>
   <td style="text-align:left;"> &lt; 0.05 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Resource guarding around dogs </td>
   <td style="text-align:left;"> Dog-directed aggression </td>
   <td style="text-align:right;"> 0.46 </td>
   <td style="text-align:left;"> &lt; 0.05 </td>
  </tr>
</tbody>
</table>

---
# BCL and C-BARQ Items: Dog-directed Aggression

<table class="table" style="margin-left: auto; margin-right: auto;">
 <thead>
  <tr>
   <th style="text-align:left;"> BCL </th>
   <th style="text-align:left;"> C-BARQ </th>
   <th style="text-align:right;"> rho </th>
   <th style="text-align:right;"> p </th>
  </tr>
 </thead>
<tbody>
  <tr>
   <td style="text-align:left;"> Dog-directed aggression </td>
   <td style="text-align:left;"> Aggression when approached directly by an unfamiliar male dog while being walked/exercised on a leash </td>
   <td style="text-align:right;"> 0.48 </td>
   <td style="text-align:right;"> 0.0003 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Dog-directed aggression </td>
   <td style="text-align:left;"> Aggression when approached directly by an unfamiliar female dog while being walked/exercised on a leash </td>
   <td style="text-align:right;"> 0.49 </td>
   <td style="text-align:right;"> 0.0001 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Dog-directed aggression </td>
   <td style="text-align:left;"> Aggression when barked, growled, or lunged at by another (unfamiliar) dog </td>
   <td style="text-align:right;"> 0.42 </td>
   <td style="text-align:right;"> 0.0110 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Resource guarding around dogs </td>
   <td style="text-align:left;"> Aggression when approached directly by an unfamiliar female dog while being walked/exercised on a leash </td>
   <td style="text-align:right;"> 0.50 </td>
   <td style="text-align:right;"> 0.0045 </td>
  </tr>
  <tr>
   <td style="text-align:left;"> Resource guarding around dogs </td>
   <td style="text-align:left;"> Aggression when barked, growled, or lunged at by another (unfamiliar) dog </td>
   <td style="text-align:right;"> 0.47 </td>
   <td style="text-align:right;"> 0.0248 </td>
  </tr>
</tbody>
</table>

---
#

# BCL and C-BARQ Items: Stranger-directed Aggression

---

# BCL and C-BARQ Items: Touch Sensitivity

---

# Behaviors with No Correlation

.pull-left[
- Trainability
- Inter-dog rivalry
- Chasing
- Excitability
- Attention-seeking
- Energy]

.pull-right[
- Owner-directed aggression
- Dog-directed fear
- Stranger-directed fear
- Non-social fear]

---

# Why don't we find high correlations?

"Dogs behave differently at home and at the Center" ----Dr. Cindy Otto

.pull-left[
<img src="img/VaukBobHammock.jpg" title="A laughing man is lying on his back in a hammock next to a squiggly black lab, paws in the air, head so far back only the underside of the jaw is visible." alt="A laughing man is lying on his back in a hammock next to a squiggly black lab, paws in the air, head so far back only the underside of the jaw is visible." width="430" height="90%" style="display: block; margin: auto;" />
]

.pull-right[
<img src="img/sam_9261.jpg" title="A yellow lab stands on the ground, mouth open, eyes squeezed shut, head thrown back, baying, with front paws up on a cement platform in what looks like a junkyard, as the camera looks down, askew, from above.  Perhaps the photographer is standing on the platform?" alt="A yellow lab stands on the ground, mouth open, eyes squeezed shut, head thrown back, baying, with front paws up on a cement platform in what looks like a junkyard, as the camera looks down, askew, from above.  Perhaps the photographer is standing on the platform?" width="389" height="99%" style="display: block; margin: auto;" />
]
---

# Why don't we find high correlations?

- Fosters and trainers have different experience and expectations
- C-BARQ items are more specific

---

# Limitations

- Data incomplete for early years
- Difficulty correlating aggression, fear, and touch sensitivity to success because of high success rate
   - Dogs have potential to fail or be successful in more than one category
   - Confounding between career chosen for dogs and trainers' assessments
   
---

# What's next?

.pull-left[
Development of working dog specific C-BARQ

Other factors affecting behavior 
- Breed
- Sex
- Discipline (detection, search, patrol)]

.pull-right[
<img src="img/CANTF3ontarioCanine.jpg" title="A yellow dog with dark eyebrows and muzzle edges peers down at the camera from above, through a crack between rough splintery slats (possibly a produce crate or pallet?) Most visible are the dark nostrils of the snout, and the brown eyes behind. Sunlight slants in across the dark slats at a steep angle. The ends of more pieces of wood are visible behind the dog. (Is this a SAR scenario?)" alt="A yellow dog with dark eyebrows and muzzle edges peers down at the camera from above, through a crack between rough splintery slats (possibly a produce crate or pallet?) Most visible are the dark nostrils of the snout, and the brown eyes behind. Sunlight slants in across the dark slats at a steep angle. The ends of more pieces of wood are visible behind the dog. (Is this a SAR scenario?)" width="90%" style="display: block; margin: auto;" />
]

---
# Thank You

.pull-left[
PennVet Working Dog Center
- Trainers
- Foster families

IWDC 2021 Organizing and Scientific Committees

Slide theme by [Dr. Silvia Canelón](https://silvia.rbind.io)

Alt-text descriptions by Dr. Angela Baldo
]

.pull-right[
<img src="img/v-litter 2-21.jpg" title="A row of eight young black labs, each wearing a different brightly colored collar, sit facing the camera.  There is slight variation among the dogs, in height and body shape. Out of focus landscape is in the background.  The foreground is also out of focus.  Only the dogs are in focus." alt="A row of eight young black labs, each wearing a different brightly colored collar, sit facing the camera.  There is slight variation among the dogs, in height and body shape. Out of focus landscape is in the background.  The foreground is also out of focus.  Only the dogs are in focus." width="90%" style="display: block; margin: auto;" />
]

???
This slide uses:
- a code chunk to produce a ggplot
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# Thank you!

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<img 
src="img/Profile_LH.JPG" 
width="200px" 
alt="Profile of Liz Hare"
style="border-radius: 50%;"/>

## Elizabeth Hare PhD

[<svg aria-label="Link" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Link</title><path d="M326.612 185.391c59.747 59.809 58.927 155.698.36 214.59-.11.12-.24.25-.36.37l-67.2 67.2c-59.27 59.27-155.699 59.262-214.96 0-59.27-59.26-59.27-155.7 0-214.96l37.106-37.106c9.84-9.84 26.786-3.3 27.294 10.606.648 17.722 3.826 35.527 9.69 52.721 1.986 5.822.567 12.262-3.783 16.612l-13.087 13.087c-28.026 28.026-28.905 73.66-1.155 101.96 28.024 28.579 74.086 28.749 102.325.51l67.2-67.19c28.191-28.191 28.073-73.757 0-101.83-3.701-3.694-7.429-6.564-10.341-8.569a16.037 16.037 0 0 1-6.947-12.606c-.396-10.567 3.348-21.456 11.698-29.806l21.054-21.055c5.521-5.521 14.182-6.199 20.584-1.731a152.482 152.482 0 0 1 20.522 17.197zM467.547 44.449c-59.261-59.262-155.69-59.27-214.96 0l-67.2 67.2c-.12.12-.25.25-.36.37-58.566 58.892-59.387 154.781.36 214.59a152.454 152.454 0 0 0 20.521 17.196c6.402 4.468 15.064 3.789 20.584-1.731l21.054-21.055c8.35-8.35 12.094-19.239 11.698-29.806a16.037 16.037 0 0 0-6.947-12.606c-2.912-2.005-6.64-4.875-10.341-8.569-28.073-28.073-28.191-73.639 0-101.83l67.2-67.19c28.239-28.239 74.3-28.069 102.325.51 27.75 28.3 26.872 73.934-1.155 101.96l-13.087 13.087c-4.35 4.35-5.769 10.79-3.783 16.612 5.864 17.194 9.042 34.999 9.69 52.721.509 13.906 17.454 20.446 27.294 10.606l37.106-37.106c59.271-59.259 59.271-155.699.001-214.959z"/></svg> doggenetics.com](http://doggenetics.com/)<br>
[<svg aria-label="Twitter" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Twitter</title><path d="M459.37 151.716c.325 4.548.325 9.097.325 13.645 0 138.72-105.583 298.558-298.558 298.558-59.452 0-114.68-17.219-161.137-47.106 8.447.974 16.568 1.299 25.34 1.299 49.055 0 94.213-16.568 130.274-44.832-46.132-.975-84.792-31.188-98.112-72.772 6.498.974 12.995 1.624 19.818 1.624 9.421 0 18.843-1.3 27.614-3.573-48.081-9.747-84.143-51.98-84.143-102.985v-1.299c13.969 7.797 30.214 12.67 47.431 13.319-28.264-18.843-46.781-51.005-46.781-87.391 0-19.492 5.197-37.36 14.294-52.954 51.655 63.675 129.3 105.258 216.365 109.807-1.624-7.797-2.599-15.918-2.599-24.04 0-57.828 46.782-104.934 104.934-104.934 30.213 0 57.502 12.67 76.67 33.137 23.715-4.548 46.456-13.32 66.599-25.34-7.798 24.366-24.366 44.833-46.132 57.827 21.117-2.273 41.584-8.122 60.426-16.243-14.292 20.791-32.161 39.308-52.628 54.253z"/></svg> @DogGeneticsLLC](https://twitter.com/DogGeneticsLLC)<br>
[<svg aria-label="GitHub" role="img" viewBox="0 0 496 512" style="height:1em;width:0.97em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>GitHub</title><path d="M165.9 397.4c0 2-2.3 3.6-5.2 3.6-3.3.3-5.6-1.3-5.6-3.6 0-2 2.3-3.6 5.2-3.6 3-.3 5.6 1.3 5.6 3.6zm-31.1-4.5c-.7 2 1.3 4.3 4.3 4.9 2.6 1 5.6 0 6.2-2s-1.3-4.3-4.3-5.2c-2.6-.7-5.5.3-6.2 2.3zm44.2-1.7c-2.9.7-4.9 2.6-4.6 4.9.3 2 2.9 3.3 5.9 2.6 2.9-.7 4.9-2.6 4.6-4.6-.3-1.9-3-3.2-5.9-2.9zM244.8 8C106.1 8 0 113.3 0 252c0 110.9 69.8 205.8 169.5 239.2 12.8 2.3 17.3-5.6 17.3-12.1 0-6.2-.3-40.4-.3-61.4 0 0-70 15-84.7-29.8 0 0-11.4-29.1-27.8-36.6 0 0-22.9-15.7 1.6-15.4 0 0 24.9 2 38.6 25.8 21.9 38.6 58.6 27.5 72.9 20.9 2.3-16 8.8-27.1 16-33.7-55.9-6.2-112.3-14.3-112.3-110.5 0-27.5 7.6-41.3 23.6-58.9-2.6-6.5-11.1-33.3 2.6-67.9 20.9-6.5 69 27 69 27 20-5.6 41.5-8.5 62.8-8.5s42.8 2.9 62.8 8.5c0 0 48.1-33.6 69-27 13.7 34.7 5.2 61.4 2.6 67.9 16 17.7 25.8 31.5 25.8 58.9 0 96.5-58.9 104.2-114.8 110.5 9.2 7.9 17 22.9 17 46.4 0 33.7-.3 75.4-.3 83.6 0 6.5 4.6 14.4 17.3 12.1C428.2 457.8 496 362.9 496 252 496 113.3 383.5 8 244.8 8zM97.2 352.9c-1.3 1-1 3.3.7 5.2 1.6 1.6 3.9 2.3 5.2 1 1.3-1 1-3.3-.7-5.2-1.6-1.6-3.9-2.3-5.2-1zm-10.8-8.1c-.7 1.3.3 2.9 2.3 3.9 1.6 1 3.6.7 4.3-.7.7-1.3-.3-2.9-2.3-3.9-2-.6-3.6-.3-4.3.7zm32.4 35.6c-1.6 1.3-1 4.3 1.3 6.2 2.3 2.3 5.2 2.6 6.5 1 1.3-1.3.7-4.3-1.3-6.2-2.2-2.3-5.2-2.6-6.5-1zm-11.4-14.7c-1.6 1-1.6 3.6 0 5.9 1.6 2.3 4.3 3.3 5.6 2.3 1.6-1.3 1.6-3.9 0-6.2-1.4-2.3-4-3.3-5.6-2z"/></svg> @LizHareDogs](https://github.com/LizHareDogs)<br>
[<svg aria-label="Envelope" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:currentColor;overflow:visible;position:relative;"><title>Envelope</title><path d="M502.3 190.8c3.9-3.1 9.7-.2 9.7 4.7V400c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V195.6c0-5 5.7-7.8 9.7-4.7 22.4 17.4 52.1 39.5 154.1 113.6 21.1 15.4 56.7 47.8 92.2 47.6 35.7.3 72-32.8 92.3-47.6 102-74.1 131.6-96.3 154-113.7zM256 320c23.2.4 56.6-29.2 73.4-41.4 132.7-96.3 142.8-104.7 173.4-128.7 5.8-4.5 9.2-11.5 9.2-18.9v-19c0-26.5-21.5-48-48-48H48C21.5 64 0 85.5 0 112v19c0 7.4 3.4 14.3 9.2 18.9 30.6 23.9 40.7 32.4 173.4 128.7 16.8 12.2 50.2 41.8 73.4 41.4z"/></svg> LizHare@DogGenetics.com](mailto:LizHare@DogGenetics.com)
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Thank you for joining me!
You can find my contact information linked on this slide if you want to get in touch, and I'm happy to take any questions.

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